Evaluation of Dengue Non Structural antigen ELISA for the diagnosis of Dengue Virus Infection in the acute phase of illness
Abstract
Introduction: Dengue viruses are a major public health problem in tropical and subtropical areas, being the cause of one of the most important mosquito-borne viral diseases. Outbreaks have also been reported from Mumbai and other parts of Maharashtra.
Aims and Objectives: (1) Detection and evaluation of Non-Structural Protein (NS1 Ag) in the sera of suspected cases of dengue virus infection. (2) Evaluation of Rapid Immunochromatographic test and ELISA for detection of IgM/IgG antibodies.
Material and Methods: An open labeled prospective study was carried out using 150 patient’s sera and 30 paired sera. The serum samples were subjected to Rapid Immunochromatographic test, ELISA and PCR tests.
Results: The sensitivity of Rapid test as compared to ELISA was 87.5% for IgM and 66.6% for IgG. Specificity for ELISA and Rapid test was 96.3% and 92.6% respectively. Sensitivity of NS1 Ag as compared to Dengue PCR was 86.66%.
Conclusion: Dengue NS1 Ag ELISA with its ability to specifically diagnose dengue in early stage of the disease with high sensitivity can prove a boon in the early treatment and management of dengue patients.
References
2. Dar L, Broor S, Sengupta S, Xess I, Seth P. The first major outbreak of dengue hemorrhagic fever in Delhi, India. Emerg Infect Dis. 1999;5 :589-90.
3. Agarwal R, Kapoor S, Nagar R, Mishra A, Mathur A. A clinical study of the patients with dengue hemorrhagic fever during the epidemic of 1996 at Lucknow, India. Southeast Asian J Trop Med Public Health. 1999;30:507-10.
4. Banik GB, Pal TK, Mandal A, Chakraborty MS. Dengue hemorrhagic fever in Calcutta. Indian Pediatr. 1994;31:685-7.
5. Kabilan L, Balsubramaniam S, Keshava SM, Thenmozhi V, Sekar G. Dengue disease spectrum among infants in the 2001 dengue epidemic in Chennai, Tamil Nadu, India. J ClinMicrobiol. 2003;41:3919-21.
6. Shah Ira, Deshpande GC, Tardeja PN. Outbreak of Dengue in Mumbai and predictive markers for Dengue Shock Syndrome. Journal of Tropical Pediatrics. 2004;50(5):301-305.
7. Karande S, Gandhi D, Kulkarni M, Renu B, Anuradha De. Concurrent outbreak of Leptospirosis and Dengue in Mumbai, India 2002. Journal of Tropical Pediatrics. Advance Access published online on April 14, 2005.
8. Thakare J, Walhekar B, Banerjee K. Hemorrhagic manifestations and encephalopathy in cases of dengue in India. Southeast Asian J Trop Med Public Health. 1996;27 (3):471-5.
9. Alcon Sophie, Talarmin A, Debruyne M, Falconar A, Deubel V And Flamand M. Enzyme Linked Immunosorbent Assay specific to Dengue Virus Type 1 Nonstructural Protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J ClinMicrobiol.2002;40 (2):376- 81.
10. Young Paul R, Hilditch PA, Bletchly C, Hallron W. An Antigen capture Enzyme Linked Immunosorbent Assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol. 2000;38(3):1053-1057.
11. Winkler, G., S. E. Maxwell, C. Ruemmler, and V. Stoller. Newly synthesized dengue-2 virus non-structural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane associated after dimerization. Virology. 1989;171:302-305.
12. Koraka, P., C. P. Burghoorn-Maas, A. Falconar, T. E. Setiati, K. Djamiatun, Groen J, and Osterhaus A.D.M.E. Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections. J. Clin. Microbiol. 2003:41:4154-4159.
13. Branch S L &Levett PN. Comparison of PanBio Dengue Duo Enzyme Linked Immunosorbent Assay (ELISA) and MRL Dengue fever virus immunoglobin M capture ELISA for diagnosis of Dengue virus infections in south east Asia. ClinDiagn Lab Immunol. 1999;6(5):705-712
14. Subhamoy Pal, DaunerAL, Mitra I, Forshey BM, Garcia P, Morrison AC, Halsey ES, Kochel TJ, Wu SL. Evaluation of Dengue NS1 Antigen Rapid Tests and ELISA Kits Using
Clinical Samples. PLOS ONE:Published: November 20, 2014.
